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admsc growth medium bullet kit  (Lonza)


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    Structured Review

    Lonza admsc growth medium bullet kit
    Mesenchymal cells modulate the effect of Hedgehog (Hh) signaling. ( A , B ) Proliferation analysis of 5-ethynyl-2’-deoxyuridine (EdU)+ MDA-MB-468 and 184B5 cells co-cultured with adipose mesenchymal stem cells <t>(ADMSC)</t> treated with Sonic hedgehog (SHH)-ligand and Hh inhibitors for 96 h. Significance was determined via Student’s t -test, * p < 0.05. ( C ) Expression levels of glioma associated oncogene family zinc finger 1 ( GLI1 ), patched1 ( PTCH1 ) and Smoothened ( SMO ) genes relative to GAPDH at 24 h post-treatment. ( D , E ) Tumor growth curves in xenografted tumors composed by MDA-MB-468 + ADMSC +/− SHH-ligand. Daily dosage with NVP or Vehicle during the last four weeks. Data show the average mean +/− SEM of nine mice per treatment. Significance was determined via ANOVA analysis comparing Vehicle (D) or SHH (E) with NVP, * p < 0.05, ** p < 0.01, **** p < 0.0001. F ) Expression levels of GLI1 , PTCH1 , and SMO relative to GAPDH in xenografted tumors using human and mouse-specific primers. Data represent mean +/− SEM of six mice per condition. No significant differences were observed by Student’s t -test. ( G , H ) Percentage of mice with visible metastases. Significance was determined by Fisher’s exact test, * p < 0.05.
    Admsc Growth Medium Bullet Kit, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/admsc+growth+medium+bullet+kit/pmc06826628-38-14-20?v=Lonza
    Average 90 stars, based on 1 article reviews
    admsc growth medium bullet kit - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "Mesenchymal Cells Support the Oncogenicity and Therapeutic Response of the Hedgehog Pathway in Triple-Negative Breast Cancer"

    Article Title: Mesenchymal Cells Support the Oncogenicity and Therapeutic Response of the Hedgehog Pathway in Triple-Negative Breast Cancer

    Journal: Cancers

    doi: 10.3390/cancers11101522

    Mesenchymal cells modulate the effect of Hedgehog (Hh) signaling. ( A , B ) Proliferation analysis of 5-ethynyl-2’-deoxyuridine (EdU)+ MDA-MB-468 and 184B5 cells co-cultured with adipose mesenchymal stem cells (ADMSC) treated with Sonic hedgehog (SHH)-ligand and Hh inhibitors for 96 h. Significance was determined via Student’s t -test, * p < 0.05. ( C ) Expression levels of glioma associated oncogene family zinc finger 1 ( GLI1 ), patched1 ( PTCH1 ) and Smoothened ( SMO ) genes relative to GAPDH at 24 h post-treatment. ( D , E ) Tumor growth curves in xenografted tumors composed by MDA-MB-468 + ADMSC +/− SHH-ligand. Daily dosage with NVP or Vehicle during the last four weeks. Data show the average mean +/− SEM of nine mice per treatment. Significance was determined via ANOVA analysis comparing Vehicle (D) or SHH (E) with NVP, * p < 0.05, ** p < 0.01, **** p < 0.0001. F ) Expression levels of GLI1 , PTCH1 , and SMO relative to GAPDH in xenografted tumors using human and mouse-specific primers. Data represent mean +/− SEM of six mice per condition. No significant differences were observed by Student’s t -test. ( G , H ) Percentage of mice with visible metastases. Significance was determined by Fisher’s exact test, * p < 0.05.
    Figure Legend Snippet: Mesenchymal cells modulate the effect of Hedgehog (Hh) signaling. ( A , B ) Proliferation analysis of 5-ethynyl-2’-deoxyuridine (EdU)+ MDA-MB-468 and 184B5 cells co-cultured with adipose mesenchymal stem cells (ADMSC) treated with Sonic hedgehog (SHH)-ligand and Hh inhibitors for 96 h. Significance was determined via Student’s t -test, * p < 0.05. ( C ) Expression levels of glioma associated oncogene family zinc finger 1 ( GLI1 ), patched1 ( PTCH1 ) and Smoothened ( SMO ) genes relative to GAPDH at 24 h post-treatment. ( D , E ) Tumor growth curves in xenografted tumors composed by MDA-MB-468 + ADMSC +/− SHH-ligand. Daily dosage with NVP or Vehicle during the last four weeks. Data show the average mean +/− SEM of nine mice per treatment. Significance was determined via ANOVA analysis comparing Vehicle (D) or SHH (E) with NVP, * p < 0.05, ** p < 0.01, **** p < 0.0001. F ) Expression levels of GLI1 , PTCH1 , and SMO relative to GAPDH in xenografted tumors using human and mouse-specific primers. Data represent mean +/− SEM of six mice per condition. No significant differences were observed by Student’s t -test. ( G , H ) Percentage of mice with visible metastases. Significance was determined by Fisher’s exact test, * p < 0.05.

    Techniques Used: Cell Culture, Expressing



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    Lonza admsc growth medium bullet kit
    Mesenchymal cells modulate the effect of Hedgehog (Hh) signaling. ( A , B ) Proliferation analysis of 5-ethynyl-2’-deoxyuridine (EdU)+ MDA-MB-468 and 184B5 cells co-cultured with adipose mesenchymal stem cells <t>(ADMSC)</t> treated with Sonic hedgehog (SHH)-ligand and Hh inhibitors for 96 h. Significance was determined via Student’s t -test, * p < 0.05. ( C ) Expression levels of glioma associated oncogene family zinc finger 1 ( GLI1 ), patched1 ( PTCH1 ) and Smoothened ( SMO ) genes relative to GAPDH at 24 h post-treatment. ( D , E ) Tumor growth curves in xenografted tumors composed by MDA-MB-468 + ADMSC +/− SHH-ligand. Daily dosage with NVP or Vehicle during the last four weeks. Data show the average mean +/− SEM of nine mice per treatment. Significance was determined via ANOVA analysis comparing Vehicle (D) or SHH (E) with NVP, * p < 0.05, ** p < 0.01, **** p < 0.0001. F ) Expression levels of GLI1 , PTCH1 , and SMO relative to GAPDH in xenografted tumors using human and mouse-specific primers. Data represent mean +/− SEM of six mice per condition. No significant differences were observed by Student’s t -test. ( G , H ) Percentage of mice with visible metastases. Significance was determined by Fisher’s exact test, * p < 0.05.
    Admsc Growth Medium Bullet Kit, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/admsc+growth+medium+bullet+kit/pmc06826628-38-14-20?v=Lonza
    Average 90 stars, based on 1 article reviews
    admsc growth medium bullet kit - by Bioz Stars, 2026-08
    90/100 stars
      Buy from Supplier

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    Mesenchymal cells modulate the effect of Hedgehog (Hh) signaling. ( A , B ) Proliferation analysis of 5-ethynyl-2’-deoxyuridine (EdU)+ MDA-MB-468 and 184B5 cells co-cultured with adipose mesenchymal stem cells (ADMSC) treated with Sonic hedgehog (SHH)-ligand and Hh inhibitors for 96 h. Significance was determined via Student’s t -test, * p < 0.05. ( C ) Expression levels of glioma associated oncogene family zinc finger 1 ( GLI1 ), patched1 ( PTCH1 ) and Smoothened ( SMO ) genes relative to GAPDH at 24 h post-treatment. ( D , E ) Tumor growth curves in xenografted tumors composed by MDA-MB-468 + ADMSC +/− SHH-ligand. Daily dosage with NVP or Vehicle during the last four weeks. Data show the average mean +/− SEM of nine mice per treatment. Significance was determined via ANOVA analysis comparing Vehicle (D) or SHH (E) with NVP, * p < 0.05, ** p < 0.01, **** p < 0.0001. F ) Expression levels of GLI1 , PTCH1 , and SMO relative to GAPDH in xenografted tumors using human and mouse-specific primers. Data represent mean +/− SEM of six mice per condition. No significant differences were observed by Student’s t -test. ( G , H ) Percentage of mice with visible metastases. Significance was determined by Fisher’s exact test, * p < 0.05.

    Journal: Cancers

    Article Title: Mesenchymal Cells Support the Oncogenicity and Therapeutic Response of the Hedgehog Pathway in Triple-Negative Breast Cancer

    doi: 10.3390/cancers11101522

    Figure Lengend Snippet: Mesenchymal cells modulate the effect of Hedgehog (Hh) signaling. ( A , B ) Proliferation analysis of 5-ethynyl-2’-deoxyuridine (EdU)+ MDA-MB-468 and 184B5 cells co-cultured with adipose mesenchymal stem cells (ADMSC) treated with Sonic hedgehog (SHH)-ligand and Hh inhibitors for 96 h. Significance was determined via Student’s t -test, * p < 0.05. ( C ) Expression levels of glioma associated oncogene family zinc finger 1 ( GLI1 ), patched1 ( PTCH1 ) and Smoothened ( SMO ) genes relative to GAPDH at 24 h post-treatment. ( D , E ) Tumor growth curves in xenografted tumors composed by MDA-MB-468 + ADMSC +/− SHH-ligand. Daily dosage with NVP or Vehicle during the last four weeks. Data show the average mean +/− SEM of nine mice per treatment. Significance was determined via ANOVA analysis comparing Vehicle (D) or SHH (E) with NVP, * p < 0.05, ** p < 0.01, **** p < 0.0001. F ) Expression levels of GLI1 , PTCH1 , and SMO relative to GAPDH in xenografted tumors using human and mouse-specific primers. Data represent mean +/− SEM of six mice per condition. No significant differences were observed by Student’s t -test. ( G , H ) Percentage of mice with visible metastases. Significance was determined by Fisher’s exact test, * p < 0.05.

    Article Snippet: Adipose mesenchymal stem cells (ADMSC) were purchased from Lonza and sustained and supplemented by ADMSC Growth Medium Bullet kit (PT-4505, Lonza, Walkersville, MD, USA).

    Techniques: Cell Culture, Expressing